Temporal dynamics of ovine airway epithelial cell differentiation at an air-liquid interface.

ABSTRACT: The respiratory tract and lungs are subject to diverse pathologies with wide-ranging implications for both human and animal welfare. The development and detailed characterization of cell culture models for studying such forms of disease is of critical importance. In recent years the use of air-liquid interface (ALI)-cultured airway epithelial cells has increased markedly, as this method of culture results in the formation of a highly representative, organotypic in vitro model system. In this study we have expanded on previous knowledge of differentiated ovine tracheal epithelial cells by analysing the progression of differentiation over an extensive time course at an ALI. We observed a pseudo-stratified epithelium with ciliation and a concurrent increase in cell layer thickness from 9 days

SUBMITTER: O'Boyle N 

PROVIDER: S-EPMC5529025 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature