Inflammatory Stroke Extracellular Vesicles Induce Macrophage Activation.

ABSTRACT: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs.EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression.EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression.Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells.

SUBMITTER: Couch Y 

PROVIDER: S-EPMC5531255 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature