Extracellular Vesicles from Naegleria fowleri Induce IL-8 Response in THP-1 Macrophage.

ABSTRACT: Extracellular vesicles (EVs) released from pathogenic protozoans play crucial roles in host-parasite communication and disease pathogenesis. Naegleria fowleri is a free-living protozoan causing primary amoebic meningoencephalitis, a fatal disease in the central nervous system. This study aims to explore the roles of N. fowleri-derived EVs (Nf-EVs) in host-pathogen interactions using the THP-1 cell line as a model. The Nf-EVs were isolated from the N. fowleri trophozoite culture supernatant using sequential centrifugation and characterized by nanoparticle tracking analysis and transmission electron microscopy. The functional roles of Nf-EVs in the apoptosis and immune response induction of THP-1 monocytes and macrophages were examined by flow cytome

SUBMITTER: Lertjuthaporn S 

PROVIDER: S-EPMC9231210 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature