5-Hydroxytryptamine 6 Receptor (5-HT₆R)-Mediated Morphological Changes via RhoA-Dependent Pathways.

ABSTRACT: The 5-HT₆R has been considered as an attractive therapeutic target in the brain due to its exclusive expression in the brain. However, the mechanistic linkage between 5-HT₆Rs and brain functions remains poorly understood. Here, we examined the effects of 5-HT₆R-mediated cell morphological changes using immunocytochemistry, Western blot, and live-cell imaging assays. Our results showed that the activation of 5-HT₆Rs caused morphological changes and increased cell surface area in HEK293 cells expressing 5-HT₆Rs. Treatment with 5-HT specifically increased RhoA-GTP activity without affecting other Rho family proteins, such as Rac1 and Cdc42. Furthermore, live-cell imaging in hippocampal neurons revealed that activation of 5-HT₆R

SUBMITTER: Rahman MA 

PROVIDER: S-EPMC5547219 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature