Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.

ABSTRACT:

Context

Quantitative changes of salivary proteins due to acute stress were detected.

Objective

To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.

Materials and methods

Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.

Results

Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.

Discussion and conclusion

Alpha-amylase and cystatins are poten

SUBMITTER: Marvin RK 

PROVIDER: S-EPMC5551674 | biostudies-literature | 2017 May - Jun

REPOSITORIES: biostudies-literature