MicroRNA profile in neosquamous esophageal mucosa following ablation of Barrett's esophagus.

ABSTRACT:

Aim

To investigate the microRNA expression profile in esophageal neosquamous epithelium from patients who had undergone ablation of Barrett's esophagus.

Methods

High throughput screening using TaqMan^® Array Human MicroRNA quantitative PCR was used to determine expression levels of 754 microRNAs in distal esophageal mucosa (1 cm above the gastro-esophageal junction) from 16 patients who had undergone ablation of non-dysplastic Barrett's esophagus using argon plasma coagulation vs pretreatment mucosa, post-treatment proximal normal non-treated esophageal mucosa, and esophageal mucosal biopsies from 10 controls without Barrett's esophagus. Biopsies of squamous mucosa were also taken from 5 cm above the pre-ablation squamo-columnar junction. Predicted mRNA targ

SUBMITTER: Sreedharan L 

PROVIDER: S-EPMC5558114 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature