The ciliary membrane-associated proteome reveals actin-binding proteins as key components of cilia.

ABSTRACT: Primary cilia are sensory, antennae-like organelles present on the surface of many cell types. They have been involved in a variety of diseases collectively termed ciliopathies. As cilia are essential regulators of cell signaling, the composition of the ciliary membrane needs to be strictly regulated. To understand regulatory processes at the ciliary membrane, we report the targeting of a genetically engineered enzyme specifically to the ciliary membrane to allow biotinylation and identification of the membrane-associated proteome. Bioinformatic analysis of the comprehensive dataset reveals high-stoichiometric presence of actin-binding proteins inside the cilium. Immunofluorescence stainings and complementary interaction proteomic analyses confirm these findings. Depolymerization of branch

SUBMITTER: Kohli P 

PROVIDER: S-EPMC5579364 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature