Na ⁺/Ca²⁺ exchangers and Orai channels jointly refill endoplasmic reticulum (ER) Ca²⁺ via ER nanojunctions in vascular endothelial cells.

ABSTRACT: We investigated the role of Na⁺/ Ca²⁺ exchange (NCX) in the refilling of endoplasmic reticulum (ER) Ca²⁺ in vascular endothelial cells under various conditions of cell stimulation and plasma membrane (PM) polarization. Better understanding of the mechanisms behind basic ER Ca²⁺ content regulation is important, since current hypotheses on the possible ultimate causes of ER stress point to deterioration of the Ca²⁺ transport mechanism to/from ER itself. We measured [Ca²⁺]_i temporal changes by Fura-2 fluorescence under experimental protocols that inhibit a host of transporters (NCX, Orai, non-selective transient receptor potential canonical (TRPC) channels, sarco/endoplasmic reticulum Ca²⁺ ATPase (SERCA),

SUBMITTER: Di Giuro CML 

PROVIDER: S-EPMC5590033 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature