Ras/MAPK Modifier Loci Revealed by eQTL in Caenorhabditis elegans.

ABSTRACT: The oncogenic Ras/MAPK pathway is evolutionarily conserved across metazoans. Yet, almost all our knowledge on this pathway comes from studies using single genetic backgrounds, whereas mutational effects can be highly background dependent. Therefore, we lack insight in the interplay between genetic backgrounds and the Ras/MAPK-signaling pathway. Here, we used a Caenorhabditis elegans RIL population containing a gain-of-function mutation in the Ras/MAPK-pathway gene let-60 and measured how gene expression regulation is affected by this mutation. We mapped eQTL and found that the majority (∼73%) of the 1516 detected cis-eQTL were not specific for the let-60 mutation, whereas most (∼76%) of the 898 detected trans-eQTL were associated with the let-60 mu

SUBMITTER: Sterken MG 

PROVIDER: S-EPMC5592943 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature