Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve.

ABSTRACT: Analysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems.The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work de

SUBMITTER: Banfi C 

PROVIDER: S-EPMC5608423 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature