Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.

ABSTRACT: Septic shock is a common medical condition with a mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators of sepsis are urgently needed. High-throughput technologies assessing circulating microRNAs represent an important tool for biomarker identification, but the blood-compartment specificity of these miRNAs has not yet been investigated. We characterized miRNA profiles from serum exosomes, total serum and blood cells (leukocytes, erythrocytes, platelets) of sepsis patients by next-generation sequencing and RT-qPCR (n = 3 × 22) and established differences in miRNA expression between blood compartments. In silico analysis was used to identify compartment-specific signalling functions of

SUBMITTER: Reithmair M 

PROVIDER: S-EPMC5618677 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature