Unveiling Chloroplast RNA Editing Events Using Next Generation Small RNA Sequencing Data.

ABSTRACT: Organellar RNA editing involves the modification of nucleotide sequences to maintain conserved protein functions, mainly by reverting non-neutral codon mutations. The loss of plastid editing events, resulting from mutations in RNA editing factors or through stress interference, leads to developmental, physiological and photosynthetic alterations. Recently, next generation sequencing technology has generated the massive discovery of sRNA sequences and expanded the number of sRNA data. Here, we present a method to screen chloroplast RNA editing using public sRNA libraries from Arabidopsis, soybean and rice. We mapped the sRNAs against the nuclear, mitochondrial and plastid genomes to confirm predicted cytosine to uracil (C-to-U) editing events and identify new editing sites in plastids. Amon

SUBMITTER: Rodrigues NF 

PROVIDER: S-EPMC5626879 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature