Protective efficacy of a Salmonella Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme-PMAP36 fusion protein in a murine model.

ABSTRACT: A Salmonella Typhimurium ghost vaccine was constructed with the use of a recombinant fusion protein consisting of lysozyme and porcine myeloid antimicrobial peptide 36 expressed by the Escherichia coli overexpression system. After confirmation of its effectiveness by transmission electron microscopy the vaccine was evaluated in a murine model. Of the 60 BALB/c mice equally divided into 4 groups, group A mice were intramuscularly inoculated with 100 μL of sterile phosphate-buffered saline, and the mice in groups B, C, and D were intramuscularly inoculated with approximately 1.0 × 10⁴, 1.0 × 10⁵, or 1.0 × 10⁶ cells of the S. Typhimurium ghost vaccine, respectively, in 100-μL amounts. The serum IgG titers against S. Typhimurium oute

SUBMITTER: Moon JY 

PROVIDER: S-EPMC5644453 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature