Functional assessment of human enhancer activities using whole-genome STARR-sequencing.

ABSTRACT:

Background

Genome-wide quantification of enhancer activity in the human genome has proven to be a challenging problem. Recent efforts have led to the development of powerful tools for enhancer quantification. However, because of genome size and complexity, these tools have yet to be applied to the whole human genome.

Results

 In the current study, we use a human prostate cancer cell line, LNCaP as a model to perform whole human genome STARR-seq (WHG-STARR-seq) to reliably obtain an assessment of enhancer activity. This approach builds upon previously developed STARR-seq in the fly genome and CapSTARR-seq techniques in targeted human genomic regions. With an improved library preparation strategy, our approach greatly increases the library complexity per unit of starting mater

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5694901 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature