Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.

ABSTRACT: Chlamydomonas reinhardtii is a unicellular green alga that has attracted interest due to its potential biotechnological applications, and as a model for algal biofuel and energy metabolism. Despite all the advantages that this unicellular alga offers, poor and inconsistent expression of nuclear transgenes remains an obstacle for basic and applied research. We used a data-mining strategy to identify highly expressed genes in Chlamydomonas whose flanking sequences were tested for the ability to drive heterologous nuclear transgene expression. Candidates identified in this search included two ribosomal protein genes, RPL35a and RPL23, and ferredoxin, FDX1, whose flanking regions including promoters, terminators and untranslated sequences could drive stable luciferase transgene expression to s

SUBMITTER: Lopez-Paz C 

PROVIDER: S-EPMC5718938 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature