TiO₂ nanoparticles cause mitochondrial dysfunction, activate inflammatory responses, and attenuate phagocytosis in macrophages: A proteomic and metabolomic insight.

ABSTRACT: Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in food and cosmetics but the health impact of human exposure remains poorly defined. Emerging evidence suggests that TiO₂ NPs may elicit immune responses by acting on macrophages. Our proteomic study showed that treatment of macrophages with TiO₂ NPs led to significant re-organization of cell membrane and activation of inflammation. These observations were further corroborated with transmission electron microscopy (TEM) experiments, which demonstrated that TiO₂ NPs were trapped inside of multi-vesicular bodies (MVB) through endocytotic pathways. TiO₂ NP caused significant mitochondrial dysfunction by increasing levels of mitochondrial reactive oxygen species (ROS), decreasing A

SUBMITTER: Chen Q 

PROVIDER: S-EPMC5752088 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature