Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system.

ABSTRACT: Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi-library screening can be accomplished in a single pool without any individual treatment. This system is based on the phiC31 integrase-mediated integration between bait and prey plasmids. The integrated fragments were digested by MmeI and subjected to deep sequencing to decode the interaction matrix

SUBMITTER: Yang F 

PROVIDER: S-EPMC5815087 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature