The Potential Protective Effect of Curcumin on Amyloid-β-42 Induced Cytotoxicity in HT-22 Cells.

ABSTRACT:

Background

We aimed to investigate the effect and mechanism of curcumin (CUR) in Alzheimer's disease (AD).

Methods

Mouse hippocampal neuronal cell line HT-22 was treated with Aβ1-42 and/or CUR, and then cell viability was evaluated by cell counting kit 8, Beclin-l level was detected using western blotting, and the formation of autophagosomes was observed by transmission electron microscopy (TEM). Furthermore, transcriptome sequencing and analysis were performed in cells with Aβ1-42 alone or Aβ1-42 + CUR.

Results

Aβ1-42 treatment significantly inhibited cell viability compared with untreated cells (P < 0.01). After treatment for 48 h, CUR remarkably promoted cell viability compared with cell treated with Aβ1-42 alone (P

SUBMITTER: Zhang L 

PROVIDER: S-EPMC5820551 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature