Clonally diverse CD38+HLA-DR+CD8+ T cells persist during fatal H7N9 disease.

ABSTRACT: Severe influenza A virus (IAV) infection is associated with immune dysfunction. Here, we show circulating CD8+ T-cell profiles from patients hospitalized with avian H7N9, seasonal IAV, and influenza vaccinees. Patient survival reflects an early, transient prevalence of highly activated CD38+HLA-DR+PD-1+ CD8+ T cells, whereas the prolonged persistence of this set is found in ultimately fatal cases. Single-cell T cell receptor (TCR)-?? analyses of activated CD38+HLA-DR+CD8+ T cells show similar TCR?? diversity but differential clonal expansion kinetics in surviving and fatal H7N9 patients. Delayed clonal expansion associated with an early dichotomy at a transcriptome level (as detected by single-cell RNAseq) is found in CD38+HLA-DR+CD8+ T cells from patients who succumbed to the disease, suggesting a divergent differentiation pathway of CD38+HLA-DR+CD8+ T cells from the outset during fatal disease. Our study proposes that effective expansion of cross-reactive influenza-specific TCR?? clonotypes with appropriate transcriptome signatures is needed for early protection against severe influenza disease.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC5827521 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature