N-linked Glycan Micro-heterogeneity in Glycoproteins of Arabidopsis.

ABSTRACT: N-glycosylation is one of the most common protein post-translational modifications in eukaryotes and has a relatively conserved core structure between fungi, animals and plants. In plants, the biosynthesis of N-glycans has been extensively studied with all the major biosynthetic enzymes characterized. However, few studies have applied advanced mass spectrometry to profile intact plant N-glycopeptides. In this study, we use hydrophilic enrichment, high-resolution tandem mass spectrometry with complementary and triggered fragmentation to profile Arabidopsis N-glycopeptides from microsomal membranes of aerial tissues. A total of 492 N-glycosites were identified from 324 Arabidopsis proteins with extensive N-glycan structural heterogeneity revealed thr

SUBMITTER: Zeng W 

PROVIDER: S-EPMC5836367 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature