Representing and Comparing Site-Specific Glycan Abundance Distributions of Glycoproteins.

ABSTRACT: A method for representing and comparing distributions of N-linked glycans located at specific sites on proteins is presented. The representation takes the form of a simple mass spectrum for a given peptide sequence, with each peak corresponding to a different glycopeptide. The mass (in place of m/z) of each peak is that of the glycan mass, and its abundance corresponds to its relative abundance in the electrospray MS¹ spectrum. This provides a facile means of representing all identifiable glycopeptides arising from a single protein "sequon" on a specific sequence, thereby enabling the comparison and searching of these distributions as routinely done for mass spectra. Likewise, these reference glycopeptide abundance distribution spectra (GADS) can be stored in searc

SUBMITTER: Remoroza CA 

PROVIDER: S-EPMC9830564 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature