Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex.

ABSTRACT: The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABA_A-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged acti

SUBMITTER: Turecek J 

PROVIDER: S-EPMC5870134 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature