Xio is a component of the Drosophila sex determination pathway and RNA N⁶-methyladenosine methyltransferase complex.

ABSTRACT: N⁶-methyladenosine (m⁶A), the most abundant chemical modification in eukaryotic mRNA, has been implicated in Drosophila sex determination by modifying Sex-lethal (Sxl) pre-mRNA and facilitating its alternative splicing. Here, we identify a sex determination gene, CG7358, and rename it xio according to its loss-of-function female-to-male transformation phenotype. xio encodes a conserved ubiquitous nuclear protein of unknown function. We show that Xio colocalizes and interacts with all previously known m⁶A writer complex subunits (METTL3, METTL14, Fl(2)d/WTAP, Vir/KIAA1429, and Nito/Rbm15) and that loss of xio is associated with phenotypes that resemble other m⁶A factors, such as sexual transf

SUBMITTER: Guo J 

PROVIDER: S-EPMC5889661 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature