LncRNA ZFAS1 as a SERCA2a Inhibitor to Cause Intracellular Ca²⁺ Overload and Contractile Dysfunction in a Mouse Model of Myocardial Infarction.

ABSTRACT:

Rationale

Ca²⁺ homeostasis-a critical determinant of cardiac contractile function-is critically regulated by SERCA2a (sarcoplasmic reticulum Ca²⁺-ATPase 2a). Our previous study has identified ZFAS1 as a new lncRNA biomarker of acute myocardial infarction (MI).

Objective

To evaluate the effects of ZFAS1 on SERCA2a and the associated Ca²⁺ homeostasis and cardiac contractile function in the setting of MI.

Methods and results

ZFAS1 expression was robustly increased in cytoplasm and sarcoplasmic reticulum in a mouse model of MI and a cellular model of hypoxia. Knockdown of endogenous ZFAS1 by virus-mediated silencing shRNA partially abrogated the ischemia-induced contractile dysfunction. Overexpression of ZFAS

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5959220 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature