The production of a recombinant tandem single chain fragment variable capable of binding prolamins triggering celiac disease.

ABSTRACT:

Background

Celiac disease (CD) is one of the most common food-related chronic disorders. It is mediated by the dietary consumption of prolamins, which are storage proteins of different grains. So far, no therapy exists and patients are bound to maintain a lifelong diet to avoid symptoms and long-term complications. To support those patients we developed a tandem single chain Fragment variable (tscFv) acting as a neutralizing agent against prolamins. We recombinantly produced this molecule in E. coli, but mainly obtained misfolded product aggregates, so-called inclusion bodies, independent of the cultivation strategy we applied.

Results

In this study, we introduce this novel tscFv against CD and present our strategy of obtaining active product from inclusion bodies. The refol

SUBMITTER: Eggenreich B 

PROVIDER: S-EPMC5975707 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature