Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi.

ABSTRACT:

Background

The human filarial parasites cause diseases that are among the most important causes of morbidity in the developing world. The elimination programs targeting these infections rely on a limited number of drugs, making the identification of new chemotherapeutic agents a high priority. The study of these parasites has lagged due to the lack of reverse genetic methods.

Methodology/principal findings

We report a novel co-culture method that results in developmentally competent infective larvae of one of the human filarial parasites (Brugia malayi) and describe a method to efficiently transfect the larval stages of this parasite. We describe the production of constructs that result in integrative transfection using the piggyBac transposon system, and a selectable marker

SUBMITTER: Liu C 

PROVIDER: S-EPMC5983866 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature