High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.

ABSTRACT: As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living m

SUBMITTER: Hillman EM 

PROVIDER: S-EPMC6002850 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature