Methanobactin from Methylosinus trichosporium OB3b inhibits N₂O reduction in denitrifiers.

ABSTRACT: Methanotrophs synthesize methanobactin, a secondary metabolite that binds copper with an unprecedentedly high affinity. Such a strategy may provide methanotrophs a "copper monopoly" that can inhibit the activity of copper-containing enzymes of other microbes, e.g., copper-dependent N₂O reductases. Here, we show that methanobactin from Methylosinus trichosporium OB3b inhibited N₂O reduction in denitrifiers. When Pseudomonas stutzeri DCP-Ps1 was incubated in cocultures with M. trichosporium OB3b or with purified methanobactin from M. trichosporium OB3b, stoichiometric N₂O production was observed from NO₃^- reduction, whereas no significant N₂O accumulation was observed in cocultures with a mutant defective in methanobactin prod

SUBMITTER: Chang J 

PROVIDER: S-EPMC6052078 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature