Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study).

ABSTRACT:

Background

High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients' clinico-pathological outcome.

Methods

A total of 222 primary (pOC) and recurrent (rOC) intra-patient paired HGSOC were assessed for immunohistochemical expression of angiogenesis-associated biomarkers (CD31, to evaluate MVD, and VEGF-A). Expression profiles were compared between pOCs and rOCs and correlated with patients' data.

Results

High intratumoural MVD and VEGF-A expression were observed in 75.7% (84/111) and 20.7% (23/111) pOCs, respectively. MVD^high and VEGF⁽⁺⁾ samples were detected in 51.4% (57/111) and 20.7% (23/111) rOCs, respectively. MVD^high/VEGF⁽⁺⁾ co-expression was found in 19.8% (22/111) and 8.1% (9/111) of pOCs and rOCs, respectively (p = 0.02). Pairwise analysis showed no significant change in MVD (p = 0.935) and VEGF-A (p = 0.121) levels from pOCs to rOCs. MVD^high pOCs were associated with higher CD3⁽⁺⁾ (p = 0.029) and CD8⁽⁺⁾ (p = 0.013) intratumoural effector TILs, while VEGF⁽⁺⁾ samples were most frequently encountered among BRCA-mutated tumours (p = 0.019). Multivariate analysis showed VEGF and MVD were not independent prognostic factors for OS.

Conclusions

HGSOC intratumoural vasculature did not undergo significant changes during disease progression. High concentration of CD31⁽⁺⁾ vessels seems to promote recruitment of effector TILs. The study also provides preliminary evidence of the correlation between VEGF-positivity and BRCA status.

SUBMITTER: Ruscito I 

PROVIDER: S-EPMC6070919 | biostudies-literature |

REPOSITORIES: biostudies-literature