Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood.

ABSTRACT:

Background

A key event in human development is the establishment of erythropoietic progenitors in the bone marrow, which is accompanied by a fetal-to-adult switch in hemoglobin expression. Understanding of this event could lead to medical application, notably treatment of sickle cell disease and β-thalassemia. The changes in gene expression of erythropoietic progenitor cells as they migrate from the fetal liver and colonize the bone marrow are still rather poorly understood, as primary fetal liver (FL) tissues are difficult to obtain.

Methods

We obtained human FL tissue and adult peripheral blood (AB) samples from Thai subjects. Primary CD34⁺ cells were cultured in vitro in a fetal bovine serum-based culture medium. After 8 days of culture, erythroid

SUBMITTER: Tangprasittipap A 

PROVIDER: S-EPMC6120446 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature