Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.

ABSTRACT: Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods. Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture. It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents. During apoptosis, a cell detaches from the extracellular matrix, degrades its internal components, and fragments its contents for easier clearance. We have developed a method that leverages this process to achieve tissue decellularization using only mild wash buffers. We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspas

SUBMITTER: Cornelison RC 

PROVIDER: S-EPMC6126930 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature