Synthetic Toolkit for Complex Genetic Circuit Engineering in Saccharomyces cerevisiae.

ABSTRACT: Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by genetically engineered cell factories. Engineering of complex metabolic routes or cell behavior control systems requires robust and predictable gene expression tools. In this challenging task, orthogonality is a fundamental prerequisite for such tools. In this study, we developed and characterized in depth a comprehensive gene expression toolkit that allows accurate control of gene expression in Saccharomyces cerevisiae without marked interference with native cellular regulation. The toolkit comprises a set of transcription factors, designed to function as synthetic activators or repressors, and transcription-factor-dependent promoters, which together provide a broad expression range surpassing

SUBMITTER: Rantasalo A 

PROVIDER: S-EPMC6150731 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature