Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography-mass spectrometry.

ABSTRACT:

Background

San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.

Methods

We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (β ₂-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.

Results

Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as β ₂-AR ligands in SAD extract. The

SUBMITTER: Fei F 

PROVIDER: S-EPMC6154427 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature