Post-stroke mRNA expression profile of MMPs: effect of genetic deletion of MMP-12.

ABSTRACT:

Background and purpose

Recent reports from our laboratory demonstrated the post-ischaemic expression profile of various matrix metalloproteinases (MMPs) in rats and the detrimental role of MMP-12 in post-stroke brain damage. We hypothesise that the post-stroke dysregulation of MMPs is similar across species and that genetic deletion of MMP-12 would not affect the post-stroke expression of other MMPs. We tested our hypothesis by determining the pre-ischaemic and post-ischaemic expression profile of MMPs in wild-type and MMP-12 knockout mice.

Methods

Focal cerebral ischaemia was induced in wild-type and MMP-12 knockout mice by middle cerebral artery occlusion procedure by insertion of a monofilament suture. One hour after ischaemia, reperfusion was initiated by removing the mo

SUBMITTER: Nalamolu KR 

PROVIDER: S-EPMC6169614 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature