Stable Frequencies of HLA-C^*03:04/Peptide-Binding KIR2DL2/3⁺ Natural Killer Cells Following Vaccination.

ABSTRACT: Inhibitory KIRs play a central role in regulating NK cell activity. KIR2DL2/3 bind to HLA-C molecules, but the modulation of these interactions by viral infections and presentation of viral epitopes is not well-understood. We investigated whether the frequencies of KIR2DL2/3⁺ NK cells recognizing HLA-C^*03:04/viral peptide complexes were impacted by YFV vaccination or HIV-1 and HCV infection. Ex vivo HLA class I tetramer staining of primary human NK cells derived from YFV-vaccinated individuals, or HIV-1- or HCV-infected individuals revealed that the YFV/HLA-C^*03:04-NS2A_4-13-tetramer bound to a larger proportion of KIR2DL2/3⁺ NK cells compared to HIV-1/HLA-C^*03:04-Gag_296-304- or HCV/HLA-C^*03:04-Co

SUBMITTER: Ziegler MC 

PROVIDER: S-EPMC6199360 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature