Immune-mediated effects targeting hepatitis C virus in a syngeneic replicon cell transplantation mouse model.

ABSTRACT:

Objective

HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells.

Design

A total of 5 million H-2^b positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2^b-restricted mice. Kinetics of tumour growth, HCV RNA replication levels and HCV-specific immune responses were monitored. For immune monitoring, new H-2^b-restricted cytotoxic T cell epit

SUBMITTER: Levander S 

PROVIDER: S-EPMC6204962 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature