Dataset Information

Differentially expressed circulating microRNAs in the development of acute diabetic Charcot foot.

ABSTRACT:

Aim

Charcot foot (CF) is a rare complication of Type 2 diabetes (T2D).

Materials & methods

We assessed circulating miRNAs in 17 patients with T2D and acute CF (G1), 17 patients with T2D (G2) and equivalent neuropathy and 17 patients with T2D without neuropathy (G3) using the high-throughput miRNA expression profiling.

Results

51 significantly deregulated miRNAs were identified in G1 versus G2, 37 in G1 versus G3 and 64 in G2 versus G3. Furthermore, we demonstrated that 16 miRNAs differentially expressed between G1 versus G2 could be involved in osteoclastic differentiation. Among them, eight are key factors involved in CF pathophysiology.

Conclusion

Our data reveal that CF patients exhibit an altered expression profile of circulating miRNAs.

SUBMITTER: Pasquier J

PROVIDER: S-EPMC6240850 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Publications

Differentially expressed circulating microRNAs in the development of acute diabetic Charcot foot.

Pasquier Jennifer J Ramachandran Vimal V Abu-Qaoud Moh'd Rasheed MR Thomas Binitha B Benurwar Manasi J MJ Chidiac Omar O Hoarau-Véchot Jessica J Robay Amal A Fakhro Khalid K Menzies Robert A RA Jayyousi Amin A Zirie Mahmoud M Al Suwaidi Jassim J Malik Rayaz A RA Talal Talal K TK Najafi-Shoushtari Seyed Hani SH Rafii Arash A Abi Khalil Charbel C

Epigenomics 20180605 10

<h4>Aim</h4>Charcot foot (CF) is a rare complication of Type 2 diabetes (T2D).<h4>Materials & methods</h4>We assessed circulating miRNAs in 17 patients with T2D and acute CF (G1), 17 patients with T2D (G2) and equivalent neuropathy and 17 patients with T2D without neuropathy (G3) using the high-throughput miRNA expression profiling.<h4>Results</h4>51 significantly deregulated miRNAs were identified in G1 versus G2, 37 in G1 versus G3 and 64 in G2 versus G3. Furthermore, we demonstrated that 16 m ...[more]

PMID: 29869523

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Project description:BackgroundMolecular changes are closely related to the pathogenesis and healing process of diabetic foot ulcers (DFUs), and are crucial for the early prediction and intervention of DFU.MethodsBioinformatics analysis was performed in this study to identify the key differentially expressed genes (DEGs) in DFU, analyze their functions and function modes, and conduct preliminary experimental verification to determine the potential pivotal genes in the pathogenesis of DFU. Two datasets, GSE68183 and GSE80178, were obtained from the Gene Expression Omnibus (GEO). DEGs were obtained using GEO2R. Six co-expressed DEGs (co-DEGs) were obtained by R language analysis. The co-DEGs were constructed by using STRING and Cytoscape 3.7.2 to construct a protein-protein interaction (PPI) network, and two hub genes, NHLRC3 and BNIP3, were identified. The BNIP3 gene was selected for further analysis. Co-DEGs were used for Gene Ontology (GO) function analysis using the WebGestalt database, and BNIP3-related biological processes focused on mitochondrial protein decomposition. GO function analysis of the BNIP3 gene and its interacting genes was carried out using the cluster profile package and org.hs.eg. db package of the R language and its biological process was enriched in the cell response to external stimuli and autophagy.ResultsBNIP3 and its interacting genes were retrieved from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and KEGG pathway enrichment analysis was performed using the WebGestalt database. The results showed that BNIP3 was significantly correlated with mitochondrial autophagy and the FoxO signaling pathway. The miRDB and TargetScan databases were used to identify the relevant microRNAs (miRNAs) regulating the BNIP3 gene, and it was found that miRNA-182 may be involved in the targeted regulation of BNIP3. Western blot analysis was performed to determine the abnormal expression of BNIP3.ConclusionsOur study found that the BNIP3 gene may be a new biomarker and intervention target for DFU.

Dataset Information

Differentially expressed circulating microRNAs in the development of acute diabetic Charcot foot.

Aim

Materials & methods

Results

Conclusion

Publications

Differentially expressed circulating microRNAs in the development of acute diabetic Charcot foot.

Similar Datasets

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