A System for Gene Expression Noise Control in Yeast.

ABSTRACT: Gene expression noise arises from stochastic variation in the synthesis and degradation of mRNA and protein molecules and creates differences in protein numbers across populations of genetically identical cells. Such variability can lead to imprecision and reduced performance of both native and synthetic networks. In principle, gene expression noise can be controlled through the rates of transcription, translation and degradation, such that different combinations of those rates lead to the same protein concentrations but at different noise levels. Here, we present a "noise tuner" which allows orthogonal control over the transcription and the mRNA degradation rates by two different inducer molecules. Combining experiments with theoretical analysis, we show that in this system the noise is l

SUBMITTER: Mundt M 

PROVIDER: S-EPMC6243393 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature