Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02.

ABSTRACT: Cyclohexylamine (CHAM) is widely used in various industries, but it is harmful to human beings and the environment. Acinetobacter sp. YT-02 can degrade CHAM via cyclohexanone as an intermediate. In this study, the cyclohexylamine oxidase (CHAO) gene from Acinetobacter sp. YT-02 was cloned. Amino acid sequence alignment indicated that the cyclohexylamine oxidase (CHAO_YT-02) was 48% identical to its homolog from Brevibacterium oxydans IH-35A (CHAO_IH-35). The enzyme was expressed in Escherichia coli BL21 (DE3), and purified to apparent homogeneity by Ni-affinity chromatography. The purified enzyme was proposed to be a dimer of molecular mass of approximately 91 kDa. The enzyme exhibited its maximum activity at 50°C and at pH 7.0. The enzyme wa

SUBMITTER: Zhou H 

PROVIDER: S-EPMC6262902 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature