Role of phage ϕ1 in two strains of Salmonella Rissen, sensitive and resistant to phage ϕ1.

ABSTRACT:

Background

The study describes the Salmonella Rissen phage ϕ1 isolated from the ϕ1-sensitive Salmonella Rissen strain R^W. The same phage was then used to select the resistant strain R^Rϕ1+, which can harbour or not ϕ1.

Results

Following this approach, we found that ϕ1, upon excision from R^W cells with mitomycin, behaves as a temperate phage: lyses host cells and generates phage particles; instead, upon spontaneous excision from R^Rϕ1+ cells, it does not generate phage particles; causes loss of phage resistance; switches the O-antigen from the smooth to the rough phenotype, and favors the transition of Salmonella Rissen from the planktonic to the biofilm growth. The R^W and R^Rϕ1+ strains differ by 10 genes; of the

SUBMITTER: Papaianni M 

PROVIDER: S-EPMC6286511 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature