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ABSTRACT: Background
The study describes the Salmonella Rissen phage ϕ1 isolated from the ϕ1-sensitive Salmonella Rissen strain RW. The same phage was then used to select the resistant strain RRϕ1+, which can harbour or not ϕ1.Results
Following this approach, we found that ϕ1, upon excision from RW cells with mitomycin, behaves as a temperate phage: lyses host cells and generates phage particles; instead, upon spontaneous excision from RRϕ1+ cells, it does not generate phage particles; causes loss of phage resistance; switches the O-antigen from the smooth to the rough phenotype, and favors the transition of Salmonella Rissen from the planktonic to the biofilm growth. The RW and RRϕ1+ strains differ by 10 genes; of these, only two (phosphomannomutase_1 and phosphomannomutase_2; both involved in the mannose synthesis pathway) display significant differences at the expression levels. This result suggests that phage resistance is associated with these two genes.Conclusions
Phage ϕ1 displays the unusual property of behaving as template as well as lytic phage. This feature was used by the phage to modulate several phases of Salmonella Rissen lifestyle.
SUBMITTER: Papaianni M
PROVIDER: S-EPMC6286511 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Papaianni Marina M Contaldi Felice F Fulgione Andrea A Woo Sheridan L SL Casillo Angela A Corsaro Maria Michela MM Parrilli Ermenegilda E Marcolungo Luca L Rossato Marzia M Delledonne Massimo M Garonzi Marianna M Iannelli Domenico D Capparelli Rosanna R
BMC microbiology 20181207 1
<h4>Background</h4>The study describes the Salmonella Rissen phage ϕ1 isolated from the ϕ1-sensitive Salmonella Rissen strain R<sup>W</sup>. The same phage was then used to select the resistant strain R<sup>R</sup>ϕ1+, which can harbour or not ϕ1.<h4>Results</h4>Following this approach, we found that ϕ1, upon excision from R<sup>W</sup> cells with mitomycin, behaves as a temperate phage: lyses host cells and generates phage particles; instead, upon spontaneous excision from R<sup>R</sup>ϕ1+ cell ...[more]