Comparison of non-magnetic and magnetic beads multiplex assay for assessment of Plasmodium falciparum antibodies.

ABSTRACT:

Background

New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead reagents for cytometric bead assays (CBA) but relative performances are not well published. We have compared two types of bead-based multiplex assays to measure relative antibody levels to malarial antigens.

Methods

Assays for the measurement of antibodies to five Plasmodium falciparum vaccine candidates using non-magnetic and magnetic fluorescent microspheres were compared for their performances with a Bio-Plex²⁰⁰ instrument. Mean fluorescence intensity (MFI) was determined from individuals

SUBMITTER: Ondigo BN 

PROVIDER: S-EPMC6321751 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature