Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.

ABSTRACT:

Aim

For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.

Materials & methods

'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.

Results

Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS. Western blotting and nanoLC-MS also revealed negative exosome markers regarding both isolation approaches.

Conclusion

The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present with

SUBMITTER: Skottvoll FS 

PROVIDER: S-EPMC6331754 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature