Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity.

ABSTRACT: Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection of cell-to-cell variation within certain cell populations. Here we report scCAT-seq, a technique for simultaneously assaying chromatin accessibility and the transcriptome within the same single cell. We show that the combined single cell signatures enable accurate construction of regulatory relationships between cis-regulatory elements and the target genes at single-cell resolution, providing a new dimension of features that helps direct discovery of regulatory patterns specific to distinct cell identities. Moreover, we generate the first single cell integrated map of chromatin accessibility and transcriptome in early embryos and demonstrate the robustness of scCAT-seq in the precise dissect

SUBMITTER: Liu L 

PROVIDER: S-EPMC6349937 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature