Quantitative Proteomics Reveals Changes in Transporter Protein Abundance in Liver, Kidney and Brain of Mice by Pregnancy.

ABSTRACT:

Background

Few studies have systematically investigated pregnancy-induced changes in protein abundance of drug transporters in organs important for drug/xenobiotic disposition.

Objective

The goal of this study was to compare protein abundance of important drug/xenobiotic transporters including Abcb1a, Abcg2, Abcc2, and Slco1b2 in the liver, kidney and brain of pregnant mice on gestation day 15 to that of non-pregnant mice.

Methods

The mass spectrometry-based proteomics was used to quantify changes in protein abundance of transporters in tissues from pregnant and non-pregnant mice.

Results

The protein levels of hepatic Abcc2, Abcc3, and Slco1a4 per μg of total membrane proteins were significantly decreased by pregnancy by 24%, 72%, and 70%, respectively. The pro

SUBMITTER: Liao MZ 

PROVIDER: S-EPMC6350206 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature