Identification of Clostridium difficile Immunoreactive Spore Proteins of the Epidemic Strain R20291.

ABSTRACT:

Purpose

Clostridium difficile infections are the leading cause of diarrhea associated with the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. The development of vaccine and passive immunization therapies against C. difficile has focused on toxins A and B. In this study, an immunoproteome-based approach to identify immunogenic proteins located on the outer layers of C. difficile spores as potential candidates for the development of immunotherapy and/or diagnostic methods against this devastating infection is used.

Experimental design

To identify potential immunogenic proteins on the surface of C. difficile R20291, spore coat/exosporium extracts are separa

SUBMITTER: Pizarro-Guajardo M 

PROVIDER: S-EPMC6370038 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature