Fermentative production of the unnatural amino acid L-2-aminobutyric acid based on metabolic engineering.

ABSTRACT:

Background

L-2-aminobutyric acid (L-ABA) is an unnatural amino acid that is a key intermediate for the synthesis of several important pharmaceuticals. To make the biosynthesis of L-ABA environmental friendly and more suitable for the industrial-scale production. We expand the nature metabolic network of Escherichia coli using metabolic engineering approach for the production of L-ABA.

Results

In this study, Escherichia coli THR strain with a modified pathway for threonine-hyperproduction was engineered via deletion of the rhtA gene from the chromosome. To redirect carbon flux from 2-ketobutyrate (2-KB) to L-ABA, the ilvIH gene was deleted to block the L-isoleucine pathway. Furthermore, the ilvA gene from Escherichia coli W3110 and the leuDH gene from Thermoactinomyces interm

SUBMITTER: Xu JM 

PROVIDER: S-EPMC6393993 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature