Mobile platform for rapid sub-picogram-per-milliliter, multiplexed, digital droplet detection of proteins.

ABSTRACT: Digital droplet assays-in which biological samples are compartmentalized into millions of femtoliter-volume droplets and interrogated individually-have generated enormous enthusiasm for their ability to detect biomarkers with single-molecule sensitivity. These assays have untapped potential for point-of-care diagnostics but are currently mainly confined to laboratory settings, due to the instrumentation necessary to serially generate, control, and measure tens of millions of droplets/compartments. To address this challenge, we developed an optofluidic platform that miniaturizes digital assays into a mobile format by parallelizing their operation. This technology is based on three key innovations: (i) the integration and parallel operation of a hundred droplet generators onto a singl

SUBMITTER: Yelleswarapu V 

PROVIDER: S-EPMC6410864 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature