Heat Shock Factor 1 Drives Intergenic Association of Its Target Gene Loci upon Heat Shock.

ABSTRACT: Transcriptional induction of heat shock protein (HSP) genes is accompanied by dynamic changes in their 3D structure and spatial organization, yet the molecular basis for these phenomena remains unknown. Using chromosome conformation capture and single-cell imaging, we show that genes transcriptionally activated by Hsf1 specifically interact across chromosomes and coalesce into diffraction-limited intranuclear foci. Genes activated by the alternative stress regulators Msn2/Msn4, in contrast, do not interact among themselves nor with Hsf1 targets. Likewise, constitutively expressed genes, even those interposed between HSP genes, show no detectable interaction. Hsf1 forms discrete subnuclear puncta when stress activated, and these puncta dissolve in concert with transcriptional attenuation, p

SUBMITTER: Chowdhary S 

PROVIDER: S-EPMC6435272 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature