Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications.

ABSTRACT: Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation. Here we compared the efficacy of four kits from Invitrogen, 101Bio, Wako and iZON along with conventional ultracentrifugation-based method for exosome yield, purity and quality. Cell culture supernatant was used as an abundant source of exosomes, and exosome quantity, size-distribution, zeta-potential, marker-expression and RNA/protein quality were determined. The Invitrogen kit gave the highest yield but the preparation showed broader size-distribution likely due to microvesicle co-precipitation and had the least dispers

SUBMITTER: Patel GK 

PROVIDER: S-EPMC6441044 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature