Longitudinal analysis of acute and convalescent B cell responses in a human primary dengue serotype 2 infection model.

ABSTRACT:

Background

Acute viral infections induce a rapid and transient increase in antibody-secreting plasmablasts. At convalescence, memory B cells (MBC) and long-lived plasma cells (LLPC) are responsible for long-term humoral immunity. Following an acute viral infection, the specific properties and relationships between antibodies produced by these B cell compartments are poorly understood.

Methods

We utilized a controlled human challenge model of primary dengue virus serotype 2 (DENV2) infection to study acute and convalescent B-cell responses.

Findings

The level of DENV2 replication was correlated with the magnitude of the plasmablast response. Functional analysis of plasmablast-derived monoclonal antibodies showed that the DENV2-specific response was dominated by cells p

SUBMITTER: Nivarthi UK 

PROVIDER: S-EPMC6444124 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature